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Rev Inst Med Trop Sao Paulo. 2009 Sep-Oct;51(5):255-9.

Histopathology, serology and cultures in the diagnosis of cryptococcosis.

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1
Doutora em Ciências Pneumológicas, Unidade de Microbiologia, Universitat Rovira i Virgili, Reus, Espanha.

Abstract

Cryptococcosis is one of the most common opportunistic fungal infections in patients with acquired immunodeficiency syndrome (AIDS). We report 13 cases of cryptococcal infection based on histopathology, serology and cultures. Epidemiological analysis, histochemical techniques of hematoxilin and eosin (HE) and Grocot's silver (GMS), as well special histochemical techniques such as Mayer's mucicarmine (MM) and Fontana-Masson (FM), cryptococcal antigen test (CrAg) and isolation on fungal media: Sabouraud's (SAB), brain-heart infusion agar (BHI) and canavanine-glycine-bromothymol blue (CGB) agar were analyzed. Unsatisfactory staining results by MM stain associated to negative titers by CrAg test, which FM stain confirmed that capsule-deficient Cryptococcus infections were observed in four cases. Eight isolated cases were identified as follows: six cases were infection with Cryptococcus neoformans and two cases were Cryptococcus gattii.

PMID:
19893977
DOI:
10.1590/s0036-46652009000500004
[Indexed for MEDLINE]
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