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Methods Enzymol. 2009;463:565-72. doi: 10.1016/S0076-6879(09)63032-9.

Elution of proteins from gels.

Author information

1
McArdle Laboratory for Cancer Research, University of Wisconsin-Madison, Madison, Wisconsin, USA.

Abstract

New protein biochemical technologies have been developed that allow one to learn more and more about a protein with less and less material (on the order of microg). Polyacrylamide gel electrophoresis, which was originally strictly an analytical method, has in some cases become a high-resolution preparative method. This chapter will focus on the elution of proteins from SDS gels, with an emphasis on recovering enzyme or biological activity of the resulting protein.

PMID:
19892192
DOI:
10.1016/S0076-6879(09)63032-9
[Indexed for MEDLINE]

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