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Curr Opin Struct Biol. 2009 Dec;19(6):701-7. doi: 10.1016/j.sbi.2009.10.002. Epub 2009 Nov 2.

Macromolecular micromovements: how RNA polymerase translocates.

Author information

1
Department of Biochemistry, New York University School of Medicine, New York, NY 10016, USA.

Abstract

Multi-subunit DNA-dependent RNA polymerases synthesize RNA molecules thousands of nucleotides long. The reiterative reaction of nucleotide condensation occurs at rates of tens of nucleotides per second, invariably linked to the translocation of the enzyme along the DNA template, or threading of the DNA and the nascent RNA molecule through the enzyme. Reiteration of the nucleotide addition/translocation cycle without dissociation from the DNA and RNA requires both isomorphic and metamorphic conformational flexibility of a magnitude substantial enough to accommodate the requisite molecular motions. Here we review some of the more recently acquired insights into the structural flexibility and morphic fluctuations of RNA polymerases and their mechanistic implications.

PMID:
19889534
PMCID:
PMC3814128
DOI:
10.1016/j.sbi.2009.10.002
[Indexed for MEDLINE]
Free PMC Article

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