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Curr Opin Neurobiol. 2009 Oct;19(5):537-43. doi: 10.1016/j.conb.2009.10.002. Epub 2009 Nov 4.

High-content screening of primary neurons: ready for prime time.

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1
Gladstone Institute of Neurological Disease, San Francisco, CA 94158, United States.

Abstract

High-content screening (HCS), historically limited to drug-development companies, is now a powerful and affordable technology for academic researchers. Through automated routines, this technology acquires large datasets of fluorescence images depicting the functional states of thousands to millions of cells. Information on shapes, textures, intensities, and localizations is then used to create unique representations, or 'phenotypic signatures,' of each cell. These signatures quantify physiologic or diseased states, for example, dendritic arborization, drug response, or cell coping strategies. Live-cell imaging in HCS adds the ability to correlate cellular events at different points in time, thereby allowing sensitivities and observations not possible with fixed endpoint analysis. HCS with live-cell imaging therefore provides an unprecedented capability to detect spatiotemporal changes in cells and is particularly suited for time-dependent, stochastic processes such as neurodegenerative disorders.

PMID:
19889533
PMCID:
PMC2787795
DOI:
10.1016/j.conb.2009.10.002
[Indexed for MEDLINE]
Free PMC Article
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