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Neurosci Res. 2010 Feb;66(2):223-7. doi: 10.1016/j.neures.2009.10.007. Epub 2009 Oct 31.

Self-sustained circadian rhythm in cultured human mononuclear cells isolated from peripheral blood.

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Department of Sleep Disorder Research (Alfressa), Graduate School of Medicine, University of Tokyo, Tokyo 113-0033, Japan. <>


Disturbed circadian rhythmicity is associated with human diseases such as sleep and mood disorders. However, study of human endogenous circadian rhythm is laborious and time-consuming, which hampers the elucidation of diseases. It has been reported that peripheral tissues exhibit circadian rhythmicity as the suprachiasmatic nucleus-the center of the biological clock. We tried to study human circadian rhythm using cultured peripheral blood mononuclear cells (PBMCs) obtained from a single collection of venous blood. Activated human PBMCs showed self-sustained circadian rhythm of clock gene expression, which indicates that they are useful for investigating human endogenous circadian rhythm.

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