Comparison of clinical features and immunological parameters of patients with dehydrating diarrhoea infected with Inaba or Ogawa serotypes of Vibrio cholerae O1

Scand J Infect Dis. 2010;42(1):48-56. doi: 10.3109/00365540903289688.

Abstract

Vibrio cholerae O1, Ogawa and Inaba serotypes, both cause severe cholera. We compared clinical and immunological features in patients in Bangladesh infected with these 2 serotypes. Blood was collected from hospitalized Ogawa (N=146) or Inaba (N=191) patients at the acute stage (day 2) and 5 and 19 days later. Ogawa patients were younger than Inaba, presented with shorter duration of diarrhoea, and had more frequent abdominal pain, vomiting and need for intravenous fluids (p<0.05). Inaba patients more frequently had dark-field positive stools (p<0.01). Inaba strains were more susceptible to tetracycline and erythromycin than Ogawa strains (p<0.001). Ogawa infection produced higher plasma vibriocidal as well as IgG responses to cholera toxin B subunit, toxin-coregulated pilus subunit and lipopolysaccharide (LPS); higher IgA responses to LPS in 'antibody in lymphocyte supernatant' (ALS) specimens were also seen. These results suggest that a cholera vaccine based on the Ogawa serotype needs to be further investigated.

Publication types

  • Comparative Study
  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adolescent
  • Adult
  • Aged
  • Aged, 80 and over
  • Anti-Bacterial Agents / pharmacology
  • Antibodies, Bacterial / blood*
  • Bangladesh
  • Child
  • Child, Preschool
  • Cholera / complications
  • Cholera / immunology*
  • Cholera / microbiology
  • Cholera / pathology*
  • Cholera Toxin / immunology
  • Dehydration / etiology*
  • Dehydration / pathology*
  • Erythromycin / pharmacology
  • Feces / microbiology
  • Female
  • Humans
  • Immunoglobulin G / blood
  • Infant
  • Lipopolysaccharides / immunology
  • Male
  • Microbial Sensitivity Tests
  • Microbial Viability
  • Middle Aged
  • Serotyping
  • Tetracycline / pharmacology
  • Vibrio cholerae O1 / classification
  • Vibrio cholerae O1 / drug effects
  • Vibrio cholerae O1 / immunology*
  • Vibrio cholerae O1 / isolation & purification*
  • Young Adult

Substances

  • Anti-Bacterial Agents
  • Antibodies, Bacterial
  • Immunoglobulin G
  • Lipopolysaccharides
  • Erythromycin
  • Cholera Toxin
  • Tetracycline