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Nat Struct Mol Biol. 2009 Nov;16(11):1192-4. doi: 10.1038/nsmb.1681. Epub 2009 Nov 1.

Regulation of a muralytic enzyme by dynamic membrane topology.

Author information

1
Department of Biochemistry and Biophysics, Texas A&M University, College Station, Texas, USA.

Abstract

R(21), the lysozyme of coliphage 21, has an N-terminal signal-anchor-release (SAR) domain that directs its secretion in a membrane-tethered, inactive form and then its release and activation in the periplasm. Both genetic and crystallographic studies show that the SAR domain, once extracted from the bilayer, refolds into the body of the enzyme and effects muralytic activation by repositioning one residue of the canonical lysozyme catalytic triad.

PMID:
19881499
PMCID:
PMC3075974
DOI:
10.1038/nsmb.1681
[Indexed for MEDLINE]
Free PMC Article

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