Immunoblot analysis of Yap1, Mob1, and Mst1/2 in human HCC. Western blot analysis of 21 paired samples of non-tumorous liver (N) and HCC (T) from the same patient. Patients 2,3,5,10,11,13 and 15 exhibit substantially lower pYap1 in the tumor relative to the non-tumorous liver. Patients 4,6,12, and 15 exhibit Yap1 polypeptide overexpression in the tumor. The majority of HCCs exhibit much lower pMob1 relative to the paired non-tumorous liver. The Mst1 34kDa polypeptide fragment is lacking in the tumors while present in the non-tumorous livers of patients 3,4,5,7,8,9,11,13,15,17,19,20,21 whereas only patients 6 and 18 exhibit detectable 34kDa Mst1 in their tumors but little or none in their non-tumorous livers. The associated clinical information is presented in .