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Endocr Res. 2009;34(4):101-8. doi: 10.3109/07435800903204082.

Effects of resin or charcoal treatment on fetal bovine serum and bovine calf serum.

Author information

1
Laboratory of Molecular Diagnostics, Wadsworth Center for Laboratories and Research, New York State Department of Health, Albany, New York, USA.

Abstract

INTRODUCTION:

Charcoal- or resin-stripping of fetal bovine serum (FBS) or bovine calf serum (BCS) intended for supplementation of cell culture media is widely practiced to remove a variety of endogenous compounds, including steroid, peptide, and thyroid hormones. The possibility that stripping removes other biologically relevant factors from serum may not be appreciated.

METHODS:

In this report, standardized clinical laboratory testing methods were used to assess the effects of resin- and charcoal-stripping on content in FBS and BCS of more than 25 analytes in the sera.

RESULTS AND CONCLUSION:

In addition to hormones, the serum constituents affected by stripping are certain vitamins, electrolytes, enzyme activities, and metabolites.

PMID:
19878070
DOI:
10.3109/07435800903204082
[Indexed for MEDLINE]

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