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Anal Bioanal Chem. 2010 Jan;396(2):775-81. doi: 10.1007/s00216-009-3228-4. Epub 2009 Oct 31.

Separation of peptides by HPLC using a surface-confined ionic liquid stationary phase.

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1
Department of Chemistry, P. O. Box 210172, Cincinnati, OH 45221-0172, USA.

Abstract

A butylimidazolium bromide surface-confined ionic liquid stationary phase was synthesized in-house. The synthesized phase was investigated for the separation of five peptides (Gly-Tyr, Val-Tyr-Val, leucine enkephalin, methionine enkephalin, and angiotensin-II). The peptides were successfully separated in less than 5 min. The effect of trifluoroacetic acid (TFA) on the separation of peptides was evaluated with results confirming that TFA was not acting as ion-pairing agent in separation of peptides on this phase.

PMID:
19876619
DOI:
10.1007/s00216-009-3228-4
[Indexed for MEDLINE]
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