Format

Send to

Choose Destination
Zhong Yao Cai. 2009 Jul;32(7):1097-101.

[The effect of gallic acid extracted from leaves of Phyllanthus emblica on apoptosis of human hepatocellular carcinoma BEL-7404 cells].

[Article in Chinese]

Author information

1
Guangxi Traditional Chinese Medical University, Nanning 530001, China. zhongzg@gxtcmu.edu.cn

Abstract

OBJECTIVE:

To explore the effect of gallic acid extracted from Leaves of Phyllanthus emblica on the apoptosis of BEL-7404 cells.

METHODS:

MTT assay was applied to detect the influence on prolifetation in vitro. Inverted microscope was utilized to observe the morphological changes after BEL-7404 cells were treated with gallic acid. Annexin V/PI double label method was used to detect earlier period apoptosis cells and Tunel was applied to calculate the apoptosis rates.

RESULTS:

Gallic acid could restrain the BEL-7404 cells proliferation at diffierent levels in a time and concentration dependent manner. The typical morphological changes of apoptosis were observed after BEL-7404 cells were treated with gallic acid. Annexin V/PI double label method and Tunel method showed that the viable apoptotic cell and apoptosis rates added as action time prolonged.

CONCLUSION:

Gallic acid can restrain the BEL-7404 cells proliferation and induce apoptosis, and its effect on apoptosis is time dependent.

PMID:
19873740
[Indexed for MEDLINE]

Supplemental Content

Loading ...
Support Center