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J Biol Inorg Chem. 2010 Jan;15(1):99-105. doi: 10.1007/s00775-009-0598-1. Epub 2009 Oct 29.

Copper redistribution in Atox1-deficient mouse fibroblast cells.

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1
Petit Institute for Bioengineering and Bioscience, Georgia Institute of Technology, Atlanta, 30332, USA.

Abstract

Quantitative synchrotron X-ray fluorescence (SXRF) imaging of adherent mouse fibroblast cells deficient in antioxidant-1 (Atox1), a metallochaperone protein responsible for delivering Cu to cuproenzymes in the trans-Golgi network, revealed striking differences in the subcellular Cu distribution compared with wild-type cells. Whereas the latter showed a pronounced perinuclear localization of Cu, the Atox1-deficient cells displayed a mostly unstructured and diffuse distribution throughout the entire cell body. Comparison of the SXRF elemental maps for Zn and Fe of the same samples showed no marked differences between the two cell lines. The data underscore the importance of Atox1, not only as a metallochaperone for delivering Cu to cuproenzymes, but also as a key player in maintaining the proper distribution and organization of Cu at the cellular level.

PMID:
19865834
PMCID:
PMC3164289
DOI:
10.1007/s00775-009-0598-1
[Indexed for MEDLINE]
Free PMC Article
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