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Fungal Genet Biol. 2010 Feb;47(2):127-42. doi: 10.1016/j.fgb.2009.10.007. Epub 2009 Oct 24.

Fungal PDR transporters: Phylogeny, topology, motifs and function.

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1
Department of Oral Sciences, University of Otago, Dunedin, New Zealand.

Abstract

The overexpression of pleiotropic drug resistance (PDR) efflux pumps of the ATP-binding cassette (ABC) transporter superfamily frequently correlates with multidrug resistance. Phylogenetic analysis of 349 full-size ( approximately 160kDa) PDR proteins (Pdrps) from 55 fungal species, including major fungal pathogens, identified nine separate protein clusters (A-G, H1a/H1b and H2). Fungal, plant and human ABCG-family Pdrps possess a nucleotide-binding domain [NBD] and a transmembrane domain [TMD] in a family-defining 'reverse' ABC transporter topology [NBD-TMD] that is duplicated [NBD-TMD](2) in full-size fungal and plant Pdrps. Although full-size Pdrps have similar halves indicating early gene duplication/fusion, they show asymmetry of their NBDs and extracellular loops (ELs). Members of cluster F are most symmetric and may be closely related to the evolutionary ancestor of Pdrps. Unique structural elements are predicted, new PDR-specific motifs identified, and the significance of these and other structural features discussed.

PMID:
19857594
PMCID:
PMC2814995
DOI:
10.1016/j.fgb.2009.10.007
[Indexed for MEDLINE]
Free PMC Article
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