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Nat Struct Mol Biol. 2009 Nov;16(11):1173-7. doi: 10.1038/nsmb.1713. Epub 2009 Oct 25.

Molecular architecture of the Nup84-Nup145C-Sec13 edge element in the nuclear pore complex lattice.

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1
Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA.

Abstract

Nuclear pore complexes (NPCs) facilitate all nucleocytoplasmic transport. These massive protein assemblies are modular, with a stable structural scaffold supporting more dynamically attached components. The scaffold is made from multiple copies of the heptameric Y complex and the heteromeric Nic96 complex. We previously showed that members of these core subcomplexes specifically share an ACE1 fold with Sec31 of the COPII vesicle coat, and we proposed a lattice model for the NPC based on this commonality. Here we present the crystal structure of the heterotrimeric 134-kDa complex of Nup84-Nup145C-Sec13 of the Y complex. The heterotypic ACE1 interaction of Nup84 and Nup145C is analogous to the homotypic ACE1 interaction of Sec31 that forms COPII lattice edge elements and is inconsistent with the alternative 'fence-like' NPC model. We construct a molecular model of the Y complex and compare the architectural principles of COPII and NPC lattices.

PMID:
19855394
PMCID:
PMC3398507
DOI:
10.1038/nsmb.1713
[Indexed for MEDLINE]
Free PMC Article
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