Format

Send to

Choose Destination
See comment in PubMed Commons below
Biotechnol J. 2010 Jan;5(1):62-74. doi: 10.1002/biot.200900146.

Statistical aspects of design and validation of microtitre-plate-based linear and non-linear parallel in vitro bioassays.

Author information

1
Roche Diagnostics GmbH, Mannheim, Germany.

Abstract

Assay validation was performed using four consecutive experiments with the related statistical evaluation. A cell-based assay on microtitre plates measured repeatedly within 1 day and on consecutive days was chosen as the model. The following problems were addressed: (i) choosing an appropriate design on a plate to avoid heterogeneities, (ii) quantification of all sources of variability and (iii) selecting between linear and non-linear parallel line assays. A mixed model was used with the random factors: rows, columns and plates and fixed effect factors with either linear or non-linear parallel line models.

PMID:
19844917
DOI:
10.1002/biot.200900146
[Indexed for MEDLINE]
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Wiley
    Loading ...
    Support Center