Format

Send to

Choose Destination
Brain Res. 2010 Jan 11;1307:43-52. doi: 10.1016/j.brainres.2009.10.024. Epub 2009 Oct 17.

A microinjection technique for targeting regions of embryonic and neonatal mouse brain in vivo.

Author information

1
Department of Neuroscience, University of Minnesota, Minneapolis, MN 55455, USA.

Abstract

A simple pressure injection technique was developed to deliver substances into specific regions of the embryonic and neonatal mouse brain in vivo. The retrograde tracers Fluorogold and cholera toxin B subunit were used to test the validity of the technique. Injected animals survived the duration of transport (24-48 h) and then were sacrificed and perfused with fixative. Small injections (<or=50 nL) were contained within targeted structures of the perinatal brain and labeled distant cells of origin in several model neural pathways. Traced neural pathways in the perinatal mouse were further examined with immunohistochemical methods to test the feasibility of double labeling experiments during development. Several experimental situations in which this technique would be useful are discussed, for example, to label projection neurons in slice or culture preparations of mouse embryos and neonates. The administration of pharmacological or genetic vectors directly into specific neural targets during development should also be feasible. An examination of the form of neural pathways during early stages of life may lead to insights regarding the functional changes that occur during critical periods of development and provide an anatomic basis for some neurodevelopmental disorders.

PMID:
19840780
PMCID:
PMC2787681
DOI:
10.1016/j.brainres.2009.10.024
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Elsevier Science Icon for PubMed Central
Loading ...
Support Center