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Anal Chem. 2009 Nov 15;81(22):9512-21. doi: 10.1021/ac901416s.

Polymerase chain reaction and immunoassay--matrix assisted laser desorption mass spectrometry using tag-mass technology: new tools to break down quantification limits and multiplexes.

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University de Lille1, CNRS, MALDI Imaging Team, Laboratoire de Neuroimmunologie des Annélides, Villeneuve d'Ascq F-59655, France.


We present a new development of the Tag-Mass concept based on a photocleavable linker with tagged molecules for polymerase chain reaction (PCR) and enzyme-linked immunosorbent assay (ELISA) quantification coupled to mass spectrometry. PCR-MS and immunosorbent assay-MS with tagged oligonucleotides, bases, and antibodies will allow the acquisition of multiplexed information from genomic, transcriptomic, and proteomic experiments. This is a novel application of Tag-Mass from tissue imaging to fluid quantification and will open doors to several clinical applications ranging from biomarker-driven gene modulation to use at the patient's bedside following treatment.

[Indexed for MEDLINE]

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