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Opt Lett. 2009 Oct 15;34(20):3154-6. doi: 10.1364/OL.34.003154.

Adaptive harmonic generation microscopy of mammalian embryos.

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1
1Department of Engineering Science, University of Oxford, Parks Road, Oxford OX1 3PJ, UK.

Abstract

Adaptive optics is implemented in a harmonic generation microscope using a wavefront sensorless correction scheme. Both the second- and third-harmonic intensity signals are used as the optimization metric. Aberration correction is performed to compensate both system- and specimen-induced aberrations by using an efficient optimization routine based upon Zernike polynomial modes. Images of live mouse embryos show an improved signal level and resolution.

PMID:
19838257
PMCID:
PMC3320044
[Indexed for MEDLINE]
Free PMC Article
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