Format

Send to

Choose Destination
Nat Methods. 2009 Nov;6(11):817-23. doi: 10.1038/nmeth.1390. Epub 2009 Oct 18.

Visual proteomics of the human pathogen Leptospira interrogans.

Author information

1
Institute of Molecular Systems Biology, The Swiss Federal Institute of Technology (ETH Zurich), Zurich, Switzerland.

Abstract

Systems biology conceptualizes biological systems as dynamic networks of interacting elements, whereby functionally important properties are thought to emerge from the structure of such networks. Owing to the ubiquitous role of complexes of interacting proteins in biological systems, their subunit composition and temporal and spatial arrangement within the cell are of particular interest. 'Visual proteomics' attempts to localize individual macromolecular complexes inside of intact cells by template matching reference structures into cryo-electron tomograms. Here we combined quantitative mass spectrometry and cryo-electron tomography to detect, count and localize specific protein complexes in the cytoplasm of the human pathogen Leptospira interrogans. We describe a scoring function for visual proteomics and assess its performance and accuracy under realistic conditions. We discuss current and general limitations of the approach, as well as expected improvements in the future.

Comment in

PMID:
19838170
PMCID:
PMC2862215
DOI:
10.1038/nmeth.1390
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Nature Publishing Group Icon for PubMed Central
Loading ...
Support Center