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Sex Transm Infect. 2010 Feb;86(1):56-60. doi: 10.1136/sti.2009.037572. Epub 2009 Oct 16.

Characterisation of Chlamydia trachomatis by ompA sequencing and multilocus sequence typing in a Swedish county before and after identification of the new variant.

Author information

1
Clinical Research Centre, Orebro University Hospital, Orebro SE-70185, Sweden. margareta.jurstrand@orebroll.se

Erratum in

  • Sex Transm Infect. 2010 Jun;86(3):250.

Abstract

OBJECTIVES:

In 2006 a new variant of Chlamydia trachomatis (nvCT), with a deletion in the cryptic plasmid, was reported in Sweden. This deletion included the targets for the genetic diagnostic systems used in many clinical laboratories and resulted in thousands of false-negative results. The aim of this study was to characterise consecutive Chlamydia tissue culture-positive samples from 2006 in Orebro County, after identification of the nvCT, and to compare the results from samples collected in the same county in 1999-2000. The study also aimed to evaluate the discriminatory capacity of multilocus sequence typing (MLST) compared with ompA sequencing.

METHODS:

ompA sequencing and MLST was used to characterise 100 consecutive Chlamydia tissue culture-positive samples.

RESULTS:

A significant (p<0.001) increase of genotype E, from 47% in 1999-2000 to 69% in 2006, was detected. All 41 nvCT isolates from 2006 displayed an identical ompA genotype E and MLST profile. Excluding the nvCT isolates, the distribution of ompA genotypes is similar to the genotyping results from 1999-2000. Among the wild-type genotype E isolates from 2006, 14 unique MLST sequence types were obtained from 26 isolates while they were identical in ompA genotyping. The discriminatory power (D) of C trachomatis strains in this material was 83.5% using the MLST system compared with 49.5% utilising ompA sequencing.

CONCLUSION:

In all, MLST enables improved studies of the molecular epidemiology of C trachomatis. All nvCT isolates from 2006 displayed an identical ompA genotype E and MLST profile, which strongly indicates a clonal spread of the nvCT.

PMID:
19837730
DOI:
10.1136/sti.2009.037572
[Indexed for MEDLINE]
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