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Mol Cell Neurosci. 2010 Jan;43(1):108-16. doi: 10.1016/j.mcn.2009.09.012. Epub 2009 Oct 24.

Regulated expression of the Ras effector Rin1 in forebrain neurons.

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Department of Biological Chemistry, Molecular Biology Institute and Jonsson Cancer Center, David Geffen School of Medicine at UCLA, 615 Charles E. Young Drive, 34609 BSRB, Los Angeles, CA 90095-1737, USA.


The Ras effector Rin1 is induced concomitant with synaptogenesis in forebrain neurons, where it inhibits fear conditioning and amygdala LTP. In epithelial cells, lower levels of Rin1 orchestrate receptor endocytosis. A 945 bp Rin1 promoter fragment was active in hippocampal neurons and directed accurate tissue-specific and temporal expression in transgenic mice. Regulated expression in neurons and epithelial cells was mediated in part by Snail transcriptional repressors: mutation of a conserved Snail site increased expression and endogenous Snai1 was detected at the Rin1 promoter. We also describe an element closely related to, but distinct from, the consensus site for REST, a master repressor of neuronal genes. Conversion to a consensus REST sequence reduced expression in both cell types. These results provide insight into regulated expression of a neuronal Ras effector, define a promoter useful in telencephalic neuron studies, and describe a novel REST site variant directing expression to mature neurons.

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