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Genome Biol. 2009;10(10):R115. doi: 10.1186/gb-2009-10-10-r115. Epub 2009 Oct 16.

Targeted next-generation sequencing of a cancer transcriptome enhances detection of sequence variants and novel fusion transcripts.

Author information

1
Genome Sequencing and Analysis Program, Broad Institute of MIT and Harvard, 320 Charles Street, Cambridge, MA 02141, USA. jlevin@broadinstitute.org

Abstract

Targeted RNA-Seq combines next-generation sequencing with capture of sequences from a relevant subset of a transcriptome. When testing by capturing sequences from a tumor cDNA library by hybridization to oligonucleotide probes specific for 467 cancer-related genes, this method showed high selectivity, improved mutation detection enabling discovery of novel chimeric transcripts, and provided RNA expression data. Thus, targeted RNA-Seq produces an enhanced view of the molecular state of a set of "high interest" genes.

PMID:
19835606
PMCID:
PMC2784330
DOI:
10.1186/gb-2009-10-10-r115
[Indexed for MEDLINE]
Free PMC Article
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