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Proteomics. 2009 Dec;9(24):5562-6. doi: 10.1002/pmic.200900505.

Improved reproducibility of reverse-phase protein microarrays using array microenvironment normalization.

Author information

1
Institute for Computational Medicine, Center for Cardiovascular Bioinformatics and Modeling, Johns Hopkins University, Baltimore, MD 21218, USA. troy@jhu.edu

Abstract

We introduce a novel experimental methodology for the reverse-phase protein microarray platform which reduces the typical measurement CV as much as 70%. The methodology, referred to as array microenvironment normalization, increases the statistical power of the platform. In the experiment, it enabled the detection of a 1.1-fold shift in prostate specific antigen concentration using approximately six technical replicates rather than the 37 replicates previously required. The improved reproducibility and statistical power should facilitate clinical implementation of the platform.

PMID:
19834915
PMCID:
PMC2922907
DOI:
10.1002/pmic.200900505
[Indexed for MEDLINE]
Free PMC Article

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