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Photosynth Res. 2010 Jan;103(1):31-45. doi: 10.1007/s11120-009-9499-y. Epub 2009 Oct 10.

Changes induced by the Pepper mild mottle tobamovirus on the chloroplast proteome of Nicotiana benthamiana.

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Department of Biochemistry, Molecular and Cellular Biology of Plants, Estación Experimental del Zaidín, (CSIC) C/Profesor Albareda no. 1, C.P. 18008 Granada, Spain.


We have analyzed the chloroplast proteome of Nicotiana benthamiana using two-dimensional gel electrophoresis and mass spectrometry followed by a database search. In order to improve the resolution of the two-dimensional electrophoresis gels, we have made separate maps for the low and the high pH range. At least 200 spots were detected. We identified 72 polypeptides, some being isoforms of different multiprotein families. In addition, changes in this chloroplast proteome induced by the infection with the Spanish strain of the Pepper mild mottle virus were investigated. Viral infection induced the down-regulation of several chloroplastidic proteins involved in both the photosynthetic electron-transport chain and the Benson-Calvin cycle.

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