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Genetics. 2009 Dec;183(4):1591-5. doi: 10.1534/genetics.109.109041. Epub 2009 Oct 12.

Analysis of Ipl1-mediated phosphorylation of the Ndc80 kinetochore protein in Saccharomyces cerevisiae.

Author information

1
Division of Basic Sciences, Fred Hutchinson Cancer Research Center, Seattle, Washington 98109, USA.

Abstract

Phosphorylation of the Ndc80 kinetochore protein by the Ipl1/Aurora B kinase reduces its microtubule binding activity in vitro. We found that kinetochore-bound Ndc80 is phosphorylated on Ipl1 sites in vivo, but this phosphorylation is not essential. Instead, we show that additional Ipl1 targets contribute to segregation and the spindle checkpoint.

PMID:
19822728
PMCID:
PMC2787442
DOI:
10.1534/genetics.109.109041
[Indexed for MEDLINE]
Free PMC Article

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