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J Org Chem. 2009 Nov 6;74(21):8476-9. doi: 10.1021/jo901809k.

A photolabile linker for the mild and selective cleavage of enriched biomolecules from solid support.

Author information

1
Center for Integrated Protein Science Munich, Department of Chemistry and Biochemistry, Ludwig-Maximilians-Universit√§t M√ľnchen, Butenandtstrasse 5-13, 81377 Munich, Germany.

Abstract

Selective release of enriched biomolecules from solid support is a desirable goal in proteomic and metabolomic studies. Here we demonstrate that photocleavage of a light-sensitive phenacyl ester bond is a suitable alternative cleavage strategy for the selective release of enriched biomolecules form avidin beads circumventing the disadvantages of conventional heat denaturation procedures.

PMID:
19821566
DOI:
10.1021/jo901809k
[Indexed for MEDLINE]

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