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Biotechnol Lett. 2010 Feb;32(2):243-8. doi: 10.1007/s10529-009-0133-z. Epub 2009 Oct 9.

Screening for conditions of enhanced production of a recombinant beta-glucanase secreted into the medium by Escherichia coli.

Author information

1
Fermentation Engineering, Faculty of Technology, Bielefeld University, 33594 Bielefeld, Germany.

Abstract

The extracellular production of a hybrid bacterial beta-glucanase using Escherichia coli was studied by using combinations of promoters of varying strength for both a beta-glucanase as the target protein and the Kil protein as the releasing factor. Four strains with different combinations of promoter strengths were cultivated in shake-flasks on four different media to assess the cross-influence of promoter and medium in a general manner. Promoters were taken from natural as well as synthetic sequences known to exhibit either weak or strong promoter strength. By far the highest extracellular glucanase activity (>200 U ml(-1)) was achieved when a strain harbouring the kil gene under control of a strong synthetic stationary-phase promoter and the glucanase gene under control of a strong synthetic constitutive promoter was cultivated on a complex medium mainly composed of casein peptone, yeast extract, and glycerol.

PMID:
19816658
DOI:
10.1007/s10529-009-0133-z
[Indexed for MEDLINE]

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