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Expert Rev Proteomics. 2009 Oct;6(5):573-83. doi: 10.1586/epr.09.74.

Emerging affinity-based techniques in proteomics.

Author information

1
Department of Chemical Engineering and Materials Science, Michigan State University, East Lansing, MI 48824-1226, USA.

Abstract

Proteomes of interest, such as the human proteome, have such complexity that no single technique is adequate for the complete analysis of the constituents. Depending on the goal (e.g., identification of a novel protein vs measurement of the level of a known protein), the tools required can vary significantly. While existing methods provide valuable information, their limitations drive the development of complementary, innovative methods to achieve greater breadth of coverage, dynamic range or specificity of analysis. We will discuss affinity-based methods and their applications, focusing on their unique advantages. In addition, we will describe emerging methods with potential value to proteomics, as well as the challenges that remain for proteomic studies.

PMID:
19811078
PMCID:
PMC3967512
DOI:
10.1586/epr.09.74
[Indexed for MEDLINE]
Free PMC Article

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