Format

Send to

Choose Destination
See comment in PubMed Commons below
Neurosci Res. 2010 Jan;66(1):62-85. doi: 10.1016/j.neures.2009.09.1709. Epub 2009 Oct 4.

Gene expression profiles in the common marmoset brain determined using a newly developed common marmoset-specific DNA microarray.

Author information

1
Safety Research Laboratories, Dainippon Sumitomo Pharma Co., Ltd., 3-1-98 Kasugade-naka, Konohana-ku, Osaka 554-0022, Japan. toshifumi-fukuoka@ds-pharma.co.jp

Abstract

To facilitate common marmoset brain research, we produced a DNA microarray with 7557 probe sets derived from the common marmoset brain. Gene expression profiles in the frontal lobe, hippocampus, cerebellum and amygdaloid nucleus were then analyzed and the top 100 probe sets expressed in each structure were compared. The three lists for the frontal lobe, hippocampus and amygdaloid nucleus were very similar but the probe sets for the cerebellum demonstrated specific differences. Some of the genes specifically expressed in cerebellum were analyzed by real-time quantitative PCR to verify the DNA microarray results. Of examined genes, 5 showed extremely strong expression in cerebellum in comparison with the other structures. The results of real-time quantitative PCR were well consistent with the microarray findings, validating our newly developed DNA microarray as a useful tool for brain research with the common marmoset.

PMID:
19808062
DOI:
10.1016/j.neures.2009.09.1709
[Indexed for MEDLINE]
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Elsevier Science
    Loading ...
    Support Center