Send to

Choose Destination
Proc Natl Acad Sci U S A. 2009 Sep 29;106(39):16550-5. doi: 10.1073/pnas.0908954106. Epub 2009 Sep 15.

Chemoenzymatic elaboration of monosaccharides using engineered cytochrome P450BM3 demethylases.

Author information

Division of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, CA 91125, USA.


Polysaccharides comprise an extremely important class of biopolymers that play critical roles in a wide range of biological processes, but the synthesis of these compounds is challenging because of their complex structures. We have developed a chemoenzymatic method for regioselective deprotection of monosaccharide substrates using engineered Bacillus megaterium cytochrome P450 (P450(BM3)) demethylases that provides a highly efficient means to access valuable intermediates, which can be converted to a wide range of substituted monosaccharides and polysaccharides. Demethylases displaying high levels of regioselectivity toward a number of protected monosaccharides were identified using a combination of protein and substrate engineering, suggesting that this approach ultimately could be used in the synthesis of a wide range of substituted mono- and polysaccharides for studies in chemistry, biology, and medicine.

[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for HighWire Icon for PubMed Central
Loading ...
Support Center