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Curr Opin Cell Biol. 2009 Dec;21(6):761-6. doi: 10.1016/j.ceb.2009.09.003. Epub 2009 Oct 1.

Quantitative proteomics: a tool to assess cell differentiation.

Author information

1
Department of Physiological Chemistry and Cancer Genomics Centre, University Medical Center Utrecht, Utrecht, The Netherlands. M.Vermeulen-3@umcutrecht.nl

Abstract

During cell differentiation, gene expression is regulated at multiple levels which is only partially captured by transcription profiling. In recent years it became increasingly clear that post-translational modifications of core histones and post-transcriptional regulation by RNA-binding proteins and microRNAs play an important role during differentiation. Recent advances in mass spectrometry-based quantitative proteomics now allow for genome-wide analyses at the protein level. This technology provides a powerful toolbox that can be used to study different levels of gene regulation and reveal their importance during development of multi-cellular organisms. We highlight recent studies and indicate how quantitative proteomics can be employed to investigate cell differentiation in the future.

PMID:
19800778
DOI:
10.1016/j.ceb.2009.09.003
[Indexed for MEDLINE]

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