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Eur J Clin Microbiol Infect Dis. 2010 Jan;29(1):119-22. doi: 10.1007/s10096-009-0816-3. Epub 2009 Oct 2.

Short term micro-evolution and PCR-detection of methicillin-resistant and -susceptible Staphylococcus aureus sequence type 398.

Author information

1
ErasmusMC, Department of Medical Microbiology and Infectious Diseases, University Medical Centre Rotterdam, Rotterdam, The Netherlands. w.vanwamel@erasmusmc.nl

Abstract

Micro-evolutionary analysis of 70 ST398 isolates by pulsed-field gel electrophoresis (PFGE) using Cfr9I revealed three sub-clones with abundant inter- and intra-sub-clone heterogeneity in spa- and SCCmec-types. In addition, we developed two specific PCRs for the detection of Staphylococcus aureus sequence type 398 (ST 398) isolates with 100% specificity and high sensitivity.

PMID:
19795142
PMCID:
PMC2797422
DOI:
10.1007/s10096-009-0816-3
[Indexed for MEDLINE]
Free PMC Article

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