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Anal Biochem. 2010 Feb 1;397(1):129-31. doi: 10.1016/j.ab.2009.09.041. Epub 2009 Sep 27.

Acquisition of chemiluminescent signals from immunoblots with a digital single-lens reflex camera.

Author information

1
Department of Molecular Biology and Biochemistry, University of California, Irvine, Irvine, CA 92697, USA.

Abstract

We found that certain mid-range consumer-level digital single-lens reflex (SLR) cameras using full-frame complementary metal oxide semiconductor (CMOS) sensors outperform X-ray film in acquiring signals from immunoblots that use enhanced chemiluminescence for detection. These cameras exhibit a sensitivity comparable to X-ray film, yet they provide a 3-fold increase in linear dynamic range and substantial cost savings over time, are more convenient to use, and eliminate the chemical waste associated with processing film.

PMID:
19788886
PMCID:
PMC2808431
DOI:
10.1016/j.ab.2009.09.041
[Indexed for MEDLINE]
Free PMC Article

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