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Anal Biochem. 2010 Jan 15;396(2):319-21. doi: 10.1016/j.ab.2009.09.042. Epub 2009 Sep 27.

Quantitation of plasma total 15-series F(2)-isoprostanes by sequential solid phase and liquid-liquid extraction.

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  • 1Linus Pauling Institute, Oregon State University, Corvallis, OR 97331, USA.


F(2)-isoprostanes are stereo- and regioisomers of prostaglandin F(2alpha) (PGF(2alpha)) and are used as biomarkers for lipid peroxidation. We modified our liquid-liquid extraction (LLE) procedure for F(2)-isoprostane analysis (Anal. Biochem. 350 (2006) 41-51) to use a combination of solid phase extraction (SPE) and LLE to produce a cleaner extract that can be easily concentrated. In addition, shortening the high-performance liquid chromatography (HPLC) separation increased peak heights in HPLC-tandem mass spectrometry (HPLC-MS/MS) analysis. Both changes increased the overall sensitivity of the assay. MS/MS analysis served to confirm the identity of specific peaks that may be better biomarkers than the commonly measured 8-iso-PGF(2alpha).

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