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Mol Cell Proteomics. 2010 Feb;9(2):336-50. doi: 10.1074/mcp.M900308-MCP200. Epub 2009 Sep 28.

Binding partner switching on microtubules and aurora-B in the mitosis to cytokinesis transition.

Author information

1
Department of Systems Biology, Harvard Medical School, Boston, Massachusetts 02115, USA.

Abstract

The cytoskeleton globally reorganizes between mitosis (M phase) and cytokinesis (C phase), which presumably requires extensive regulatory changes. To reveal these changes, we undertook a comparative proteomics analysis of cells tightly drug-synchronized in each phase. We identified 25 proteins that bind selectively to microtubules in C phase and identified several novel binding partners including nucleolar and spindle-associated protein. C phase-selective microtubule binding of many of these proteins depended on activity of Aurora kinases as assayed by treatment with the drug VX680. Aurora-B binding partners switched dramatically between M phase to C phase, and we identified several novel C phase-selective Aurora-B binding partners including PRC1, KIF4, and anaphase-promoting complex/cyclosome. Our approach can be extended to other cellular compartments and cell states, and our data provide the first broad biochemical framework for understanding C phase. Concretely, we report a central role for Aurora-B in regulating the C phase cytoskeleton.

PMID:
19786723
PMCID:
PMC2830844
DOI:
10.1074/mcp.M900308-MCP200
[Indexed for MEDLINE]
Free PMC Article

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