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J Pediatr. 2010 Jan;156(1):26-8. doi: 10.1016/j.jpeds.2009.07.023.

Refrigerator storage of expressed human milk in the neonatal intensive care unit.

Author information

1
Division of Neonatal-Perinatal Medicine, Schneider Children's Hospital, New Hyde Park, NY, USA.

Abstract

OBJECTIVE:

To provide recommendations for refrigerator storage of human milk, the overall integrity (bacterial growth, cell counts, and component concentrations) of milk was examined during 96 hours of storage at 4 degrees C.

STUDY DESIGN:

Fresh milk samples (n = 36) were divided and stored at 4 degrees C for 0, 24, 48, 72, and 96 hours. At each time, pH, white cell count, and osmolality were measured and additional samples were stored at -80 degrees C until analyzed for bacteria and concentrations of lactoferrin, secretory (s)IgA, fat, fatty acids, and protein.

RESULTS:

There were no significant changes for osmolality, total and Gram-negative bacterial colony counts or concentrations of sIgA, lactoferrin, and fat. Gram-positive colony counts (2.9 to 1.6 x 10(5) colony-forming units per mL), pH (7.21 to 6.68), white blood cell counts (2.31 to 1.85 x 10(6) cells per mL), and total protein (17.5 to 16.7 g/L) declined, and free fatty acid concentrations increased (0.35 to 1.28 g/L) as storage duration increased, P < .001.

CONCLUSIONS:

Changes were minimal and the overall integrity of milk during refrigerator storage was preserved. Fresh mother's milk may be stored at refrigerator temperature for as long as 96 hours.

PMID:
19783003
DOI:
10.1016/j.jpeds.2009.07.023
[Indexed for MEDLINE]

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