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J Biophotonics. 2010 Jan;3(1-2):34-43. doi: 10.1002/jbio.200910062.

Scoring of collagen organization in healthy and diseased human dermis by multiphoton microscopy.

Author information

1
L.E.N.S. and Department of Physics, University of Florence, Sesto Fiorentino, Italy. rikka@lens.unifi.it

Abstract

We have used nonlinear imaging to evaluate collagen organization in connective tissue ex-vivo samples. Image analysis methods were tested on healthy dermis, normal scars, and keloids. The evaluation of the second harmonic to autofluorescence aging index of dermis (SAAID) has allowed a first characterization of tissues by scoring the collagen/elastin content. Further analyses on collagen morphology in healthy dermis and keloids were performed by image-pattern analysis of SHG images. The gray-level co-occurrence matrix (GLCM) analysis method has allowed classification of different tissues based on the evaluation of geometrical arrangement of collagen fibrillar bundles, whereas a pattern analysis of the FFT images has allowed the discrimination of different tissues based on the anisotropy of collagen fibers distribution. This multiple scoring method represents a promising tool to be extended to other collagen disorders, as well as to be used in in-vivo skin-imaging applications.

PMID:
19771581
DOI:
10.1002/jbio.200910062
[Indexed for MEDLINE]

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