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Methods Mol Biol. 2009;578:345-61. doi: 10.1007/978-1-60327-411-1_21.

SNP detection using trityl mass tags.

Author information

1
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Miklukho-Maklaya, Moscow, Russia.

Abstract

A new method suitable for single nucleotide polymorphism (SNP) detection using differential oligonucleotide probe extension has been developed. Sulfur-linked laser-cleavable trityl labels are implemented in this protocol. The method is based on mass spectrometry and utilizes a single surface for affinity purification of extended probes and matrix-independent desorption-ionization of the cleavable labels. The usefulness of this method for SNP genotyping is demonstrated.

PMID:
19768604
DOI:
10.1007/978-1-60327-411-1_21
[Indexed for MEDLINE]

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