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Analyst. 2009 Oct;134(10):2147-52. doi: 10.1039/b913195g. Epub 2009 Aug 20.

Smoothly shifting fluorescent windows: a tunable "off-on-off" micellar sensor for pH.

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Università di Pavia, Dipartimento di Chimica Generale, viale Taramelli, 12, 27100, Pavia, Italy.


The position of the window in an "off-on-off" fluorescent pH sensor may be shifted at will along a pH axis by changing the overall charge of the micellar container. This is obtained by using non-ionic Triton X-100 as the surfactant, and by increasing the molar fraction of the anionic sodium dodecyl sulfate (chi(SDS)) as co-surfactant, with pyrene as the fluorophore and a lipophilic tertiary amine and a lipophilic pyridine as pH-switchable quenchers. As the negative micellar charge increases by increasing chi(SDS), the observed pKa of the protonated bases move to higher pH values, followed by the dumb-bell shaped "off-on-off" fluorescence intensity vs. pH profile.

[Indexed for MEDLINE]

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