Format

Send to

Choose Destination
See comment in PubMed Commons below
Curr Opin Struct Biol. 2009 Oct;19(5):596-604. doi: 10.1016/j.sbi.2009.08.003. Epub 2009 Sep 16.

Stability effects of mutations and protein evolvability.

Author information

  • 1Department of Biological Chemistry, Weizmann Institute of Science, Rehovot 76100, Israel.

Abstract

The past several years have seen novel insights at the interface of protein biophysics and evolution. The accepted paradigm that proteins can tolerate nearly any amino acid substitution has been replaced by the view that the deleterious effects of mutations, and especially their tendency to undermine the thermodynamic and kinetic stability of protein, is a major constraint on protein evolvability--the ability of proteins to acquire changes in sequence and function. We summarize recent findings regarding how mutations affect protein stability, and how stability affects protein evolution. We describe ways of predicting and analyzing stability effects of mutations, and mechanisms that buffer or compensate for these destabilizing effects and thereby promote protein evolvabilty, in nature and in the laboratory.

PMID:
19765975
DOI:
10.1016/j.sbi.2009.08.003
[PubMed - indexed for MEDLINE]
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Elsevier Science
    Loading ...
    Support Center