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Traffic. 2009 Nov;10(11):1579-84. doi: 10.1111/j.1600-0854.2009.00980.x. Epub 2009 Sep 2.

Live cell multicolor imaging of lipid droplets with a new dye, LD540.

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Max Planck Institute of Molecular Cell Biology and Genetics, Pfotenhauerstr. 108, 01307 Dresden, Germany.


A lipophilic dye based on the Bodipy fluorophore, LD540, was developed for microscopic imaging of lipid droplets. In contrast to previous lipid droplet dyes, it can spectrally be resolved from both green and red fluorophores allowing multicolor imaging in both fixed and living cells. Its improved specificity, brightness and photostability support live cell imaging, which was used to demonstrate by two-color imaging lipid droplet motility along microtubules.

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