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Methods Mol Biol. 2009;579:127-59. doi: 10.1007/978-1-60761-322-0_7.

Lipidomics of the red cell in diagnosis of human disorders.

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1
Life Sciences, King's College, London, UK.

Abstract

Applications of tandem mass spectrometry in the field of lipid clinical chemistry are considered. Haemato-logical and biochemical advantages are presented favoring the choice of red blood cell membranes as a starting material in a wide variety of biomedical fields. Practical considerations are discussed with respect to methods of sampling, storage, and lipid extraction of red blood cells. The chapter describes the capabilities of a direct infusion of raw lipid extracts in the electro-spray ionization source compared with the more sophisticated method of high-performance liquid chromatography coupled with hybrid tandem mass spectrometry. Both methods have been evaluated and have been shown to be suitable for diagnosis and/or monitoring for a variety of human disorders.

PMID:
19763474
DOI:
10.1007/978-1-60761-322-0_7
[Indexed for MEDLINE]

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