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Pediatr Blood Cancer. 2010 Jan;54(1):62-70. doi: 10.1002/pbc.22261.

Detection of residual B precursor lymphoblastic leukemia by uniform gating flow cytometry.

Author information

1
CLIP-Childhood Leukemia Investigation Prague, Prague, Czech Republic.

Abstract

BACKGROUND:

Residual disease (RD) is an important prognostic factor in acute lymphoblastic leukemia (ALL). Flow cytometry (FC)-based RD detection is easy to perform, but interpretation requires expert analysis due to individual differences among patients.

PROCEDURE:

We focused at the design of standardized and reproducible RD monitoring in ALL. RD was investigated by a uniform gating strategy, which was designed internationally and tested in one center by Ig/TCR rearrangements.

RESULTS:

For each gate, positivity cutoff value was assigned using quantification of non-leukemic background. Comparing to Ig/TCR at 0.1% level, 80 of 103 specimens were correctly diagnosed by FC. The predictive value of FC RD at day 15 was then analyzed. In B lineage ALL, day 15 FC significantly correlated with Ig/TCR results at day 33 and/or week 12 (P < 0.01). No significant correlation was found in T lineage ALL.

CONCLUSIONS:

Thus, FC with preset uniform gating at day 15 predicts PCR-detectable MRD in B precursor ALL. Presented data may be used to define new polychromatic cytometric diagnostics of MRD including semiautomatic assessment. Pediatr Blood Cancer 2010; 54:62-70. (c) 2009 Wiley-Liss, Inc.

PMID:
19760767
DOI:
10.1002/pbc.22261
[Indexed for MEDLINE]

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