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Comb Chem High Throughput Screen. 2009 Sep;12(8):791-800.

Label-free detection of biomolecular interactions using BioLayer interferometry for kinetic characterization.

Author information

1
ForteBio, Inc, CA 94025, USA. concepcionjoy@netscape.net

Abstract

The analysis of biomolecular interactions is key in the drug development process. Label-free biosensor methods provide information on binding, kinetics, concentration, and the affinity of an interaction. These techniques provide real-time monitoring of interactions between an immobilized ligand (such as a receptor) to an analyte in solution without the use of labels. Advances in biosensor design and detection using BioLayer Interferometry (BLI) provide a simple platform that enables label-free monitoring of biomolecular interactions without the use of flow cells. We review the applications of BLI in a wide variety of research and development environments for quantifying antibodies and proteins and measuring kinetics parameters.

PMID:
19758119
DOI:
10.2174/138620709789104915
[Indexed for MEDLINE]

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