Format

Send to

Choose Destination
See comment in PubMed Commons below
J Histochem Cytochem. 2009 Dec;57(12):1099-101. doi: 10.1369/jhc.2009.954867. Epub 2009 Sep 15.

The dark side of the immunohistochemical moon: industry.

Author information

1
Department of Immunocytochemistry and Elispot Assays, R&D Systems, Inc., 614 McKinley Place N.E., Minneapolis, MN 55413, USA. alex.kalyuzhny@rndsystems.com

Abstract

Modern biological research is dependent on tools developed and provided by commercial suppliers, and antibodies for immunohistochemistry are among the most frequently used of these tools. Not all commercial antibodies perform as expected, however; this problem leads researchers to waste time and money when using antibodies that perform inadequately. Different commercial suppliers offer antibodies of varying degrees of quality and, in some cases, are unable to provide expert technical support for the immunohistochemical use of their antibodies. This article briefly describes the production of commercial antibodies from the manufacturer's perspective and presents some guidelines for choosing appropriate commercial antibodies for immunohistochemistry. Additionally, the article suggests steps to establish mutually beneficial relationships between commercial antibody suppliers and researchers who use them.

PMID:
19755714
PMCID:
PMC2778082
DOI:
10.1369/jhc.2009.954867
[Indexed for MEDLINE]
Free PMC Article
PubMed Commons home

PubMed Commons

0 comments

    Supplemental Content

    Full text links

    Icon for Atypon Icon for PubMed Central
    Loading ...
    Support Center