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J Plant Physiol. 2010 Feb 15;167(3):238-41. doi: 10.1016/j.jplph.2009.08.006. Epub 2009 Sep 5.

Involvement of nitric oxide in the inhibition of nitrogenase activity by nitrate in Lotus root nodules.

Author information

1
Graduate School of Agricultural Science, Tohoku University, Sendai, Miyagi 981-8555, Japan. kazukato@alumni.tohoku-university.jp

Abstract

Nitrogenase activity, as acetylene-reduction activity (ARA), in Lotus root nodules was clearly inhibited 27h after the addition of nitrate. Nitric oxide (NO) production was detected at that time in nitrate-supplied root nodules using the NO-reactive fluorescent probe diaminofluorescein-2 diacetate. The involvement of NO production in the inhibition of nitrogenase activity by nitrate was investigated using the NO donor sodium nitroprusside (SNP) and the NO scavenger 2-(4-carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide (c-PTIO). SNP inhibited ARA at 1mM, and c-PTIO suppressed the inhibition of ARA by nitrate. These results suggest that NO is involved in the inhibition of nitrogenase activity by nitrate in Lotus root nodules.

PMID:
19733934
DOI:
10.1016/j.jplph.2009.08.006
[Indexed for MEDLINE]

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