Format

Send to

Choose Destination
Biochem Biophys Res Commun. 2009 Dec 18;390(3):394-8. doi: 10.1016/j.bbrc.2009.09.003. Epub 2009 Sep 4.

Effect of vitamin C depletion on age-related hearing loss in SMP30/GNL knockout mice.

Author information

1
Department of Otolaryngology and Head and Neck Surgery, University of Tokyo, Tokyo 113-8655, Japan.

Abstract

Using senescence marker protein 30 (SMP30)/gluconolactonase (GNL) knockout (KO) mice, which cannot synthesize vitamin C (VC), we examined whether modulating VC level affects age-related hearing loss (AHL). KO and wild-type (WT) C57BL/6 mice were given water containing 1.5 g/L VC [VC(+)] or 37.5mg/L VC [VC(-)]. At 10 months of age, KO VC(-) mice showed significant reduction in VC level in the inner ear, plasma, and liver, increase in auditory brainstem response (ABR) thresholds, and decrease in the number of spiral ganglion cells compared to WT VC(-), WT VC(+), and KO VC(+) mice. There were no differences in VC level in the inner ear, ABR thresholds, or the number of spiral ganglion cells among WT VC(-), WT VC(+), and KO VC(+) mice. These findings suggest that VC depletion can accelerate AHL but that supplementing VC may not increase VC level in the inner ear or slow AHL in mice.

PMID:
19733551
DOI:
10.1016/j.bbrc.2009.09.003
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center