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Bioelectrochemistry. 2010 Feb;77(2):139-44. doi: 10.1016/j.bioelechem.2009.08.001. Epub 2009 Aug 12.

Layer-by-Layer self-assembled acetylcholinesterase/PAMAM-Au on CNTs modified electrode for sensing pesticides.

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Department of Chemistry, East China Normal University, Shanghai, PR China.


In this paper, an acetylcholinesterase (AChE)/dendrimers polyamidoamine (PAMAM)-Au/Carbon nanotubes (CNTs) multilayer modified electrode based on LbL self-assembled technique was employed in the detection of carbofuran in samples. The configuration of the nanostructure on the electrode provided a favorable environment to the immobilization of AChE. The modified films also improved the electrocatalytic characteristics and electron transfer speed between the films and the surface of electrode. The PAMAM-Au nanoparticles were characterized by SEM and UV-VIS methods. A set of experimental conditions were also optimized for the detection of the pesticides. A linear response over carbofuran concentration in the range of 4.8x10(-9)M to 0.9x10(-7)M was exhibited with a detection limit of 4.0x10(-9)M. The biosensor showed high sensitivity, good stability and reproducibility with promising application.

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