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Photosynth Res. 2009 Oct;102(1):95-7. doi: 10.1007/s11120-009-9493-4.

Modification of a French pressure cell to improve microbial cell disruption.

Author information

1
Department of Microbiology and Immunology, University of British Columbia, 2350 Health Sciences Mall, Vancouver, BC, Canada.

Abstract

A procedure for modification of the valve stem of a 40 K French pressure cell is described. The modification should be done by a machinist and requires a metalworking lathe. After modification of the valve stem, a torlon 4203 plastic ball is used between the valve stem and valve seat to control the pressure within the cell. The torlon plastic ball is a key component needed to obtain the high pressures required for efficient disruption of microbial cells.

PMID:
19731071
DOI:
10.1007/s11120-009-9493-4
[Indexed for MEDLINE]

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