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Diagn Microbiol Infect Dis. 2009 Nov;65(3):339-44. doi: 10.1016/j.diagmicrobio.2009.07.009. Epub 2009 Sep 2.

A multiplatform real-time polymerase chain reaction detection assay for Vibrio cholerae.

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Centres for Military Medicine and for Biological Threat Preparedness, FI-00290 Helsinki, Finland.


We report a multiplatform real-time polymerase chain reaction methodology based on genes encoding for the regulatory toxR activator and enterotoxin A protein to determine enterotoxigenic Vibrio cholerae types from other vibrios. This assay, which was successfully validated on a collection of 87 bacterial strains, including 63 representatives of V. cholerae and 8 noncholera vibrios provides a rapid tool for detection and identification of cholera.

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